Coding
Part:BBa_K4060021
Designed by: Yan-Zhen, Chen Group: iGEM21_NYCU-Taipei (2021-09-30)
pelB leader sequence with aprN (without native signal peptide sequence)
aprN (without natural singal peptide) + pelB. pelB leader sequence which is a gene fragment of pelB can help to direct to E. coli periplasmic membrane. The aprN gene is the gene of Nattokinase. Nattokinase (subtilisin NAT) is a kind of extracellular alkaline serine protease, which has the ability to prevent deep vein thrombosis (DVT) due to its fibrinolytic activity.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 67
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 67
Illegal NotI site found at 211 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 67
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 67
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 345 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 349
[edit]
Categories
Parameters
None |